Deep inelastic scattering (DIS) is the process of probing the internal structure of nucleons by scattering high-energy leptons off them. The observation of Bjorken scaling -- that structure functions depend on the dimensionless ratio x = Q^2/(2M*nu) rather than on Q^2 and nu independently -- provided the first direct evidence that protons contain point-like constituents (partons), confirming the quark model.
Deep inelastic scattering was the experimental breakthrough that revealed the quark substructure of the proton. In the late 1960s, experiments at SLAC scattered high-energy electrons off protons and observed that the cross section remained large even at high momentum transfer Q^2 -- behavior characteristic of scattering off point-like objects, not a diffuse charge distribution. This was the proton analog of Rutherford scattering: just as alpha particles revealed the nucleus inside the atom, high-energy electrons revealed quarks inside the proton.
The kinematics of DIS are described by two independent variables: the momentum transfer squared Q^2 = -q^2 (the "resolution" of the virtual photon probe) and the energy transfer nu = E - E' (the energy lost by the electron). Bjorken's insight was that at large Q^2, the structure functions depend only on the dimensionless ratio x = Q^2/(2M*nu), not on Q^2 and nu independently. This Bjorken scaling implies that the electron is scattering elastically off point-like constituents -- partons -- each carrying a fraction x of the proton's momentum. The structure functions then measure the parton distribution functions: F_2(x) = sum_i e_i^2 x f_i(x), where f_i(x) is the probability of finding parton i with momentum fraction x and e_i is its charge.
The parton model reveals that the proton is far more complex than three valence quarks. At low x, the proton contains a "sea" of virtual quark-antiquark pairs and gluons, continuously created and annihilated by QCD interactions. Gluons carry about half the proton's momentum but are invisible to the electromagnetic probe (they are neutral). The evidence for gluons came from the momentum sum rule: integrating x*f(x) over all quark flavors gives only ~50% of the proton momentum, with the remainder attributed to gluons. Direct evidence for gluons followed from three-jet events at PETRA in 1979.
QCD predicts specific scaling violations -- logarithmic Q^2 dependence of the structure functions described by the DGLAP (Dokshitzer-Gribov-Lipatov-Altarelli-Parisi) evolution equations. As Q^2 increases, the virtual photon resolves finer structure: gluon radiation produces more quark-antiquark pairs at low x while depleting quarks at high x. The quantitative agreement between measured scaling violations and DGLAP predictions over four decades in Q^2 is one of the most precise tests of QCD and earned the 2004 Nobel Prize for the discovery of asymptotic freedom.