Cytokinesis is the physical division of the cytoplasm that follows mitosis (or meiosis), producing two separate daughter cells. In animal cells, a contractile ring of actin filaments forms a cleavage furrow that pinches the cell in two. In plant cells, a cell plate forms between the daughter nuclei and expands outward to form new cell walls, because the rigid cell wall prevents pinching. Cytokinesis is distinct from mitosis: nuclear division (mitosis) can occur without cytokinesis, producing multinucleate cells (syncytia).
Compare animal vs. plant cytokinesis side-by-side: mechanism, direction of division (inward furrow vs. outward plate), and structural materials used. Consider why plants cannot use a cleavage furrow (cell wall rigidity).
You have just studied mitosis — the process by which the cell divides its duplicated chromosomes into two identical sets, each enclosed in its own nuclear envelope by the end of telophase. But at the end of mitosis, you still have one cell with two nuclei. Cytokinesis is the physical act of splitting that single cell into two separate daughter cells, each with its own nucleus, cytoplasm, and organelles. It typically begins during anaphase or telophase and completes shortly after mitosis ends.
In animal cells, cytokinesis works by constriction from the outside in. A ring of actin and myosin II filaments assembles just beneath the plasma membrane at the cell's equator, positioned by signals from the mitotic spindle (specifically, the central spindle and astral microtubules, which define the division plane). This contractile ring functions like a drawstring on a bag: myosin II motor proteins slide along the actin filaments, generating force that progressively pinches the membrane inward, creating a visible indentation called the cleavage furrow. The furrow deepens until the cell is connected by only a thin bridge (the midbody), which is then severed in a final step called abscission. The entire process depends on the cell membrane being flexible enough to deform — a property that comes from its fluid phospholipid bilayer structure, which you studied as a prerequisite.
Plant cells face a fundamentally different engineering problem: they are surrounded by a rigid cell wall that cannot be pinched inward. Instead of constricting from the outside, plant cytokinesis builds a new wall from the inside out. Vesicles derived from the Golgi apparatus, carrying cell wall materials (pectins, hemicelluloses) and new membrane, are transported along remnant spindle microtubules to the center of the cell, where they fuse to form the cell plate. The cell plate expands outward toward the existing cell walls, eventually fusing with them to create a complete septum that divides the cell in two. Each side of the cell plate becomes the new plasma membrane for its respective daughter cell, and the material between them matures into the new cell wall.
An important conceptual point is that cytokinesis and mitosis are mechanistically independent. Mitosis divides the genome; cytokinesis divides the cell body. If cytokinesis fails while mitosis succeeds, the result is a single cell with two (or more) nuclei — a syncytium or multinucleate cell. This is not always pathological: skeletal muscle fibers are multinucleate syncytia formed by the intentional fusion (not failed division) of myoblasts, and some fungi grow as coenocytic hyphae with many nuclei sharing one continuous cytoplasm. Understanding that nuclear division and cytoplasmic division are separable processes clarifies many phenomena in both normal development and disease.