Difference-in-Differences in Biostatistics

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difference-in-differences DiD parallel-trends natural-experiment policy-evaluation

Core Idea

Difference-in-differences (DiD) estimates causal effects by comparing the change in outcomes over time between a group affected by a treatment or policy (treatment group) and a group not affected (control group). The treatment effect is the difference in the before-to-after change between groups: (Y_treatment_after - Y_treatment_before) - (Y_control_after - Y_control_before). DiD removes both time-invariant group differences (the treatment group may have been sicker all along) and common time trends (both groups may have been improving). The critical assumption is parallel trends: in the absence of treatment, both groups would have experienced the same change over time. DiD is widely used in health policy evaluation — assessing the effects of smoking bans, Medicaid expansions, or new hospital regulations — because these policies create natural experiments where randomization is impossible.

Explainer

Many of the most important questions in health policy cannot be studied with randomized trials. You cannot randomly assign states to expand Medicaid, randomly impose smoking bans, or randomly close hospitals. But these policy changes create natural experiments — situations where some populations are exposed to a policy and others are not, with the timing and location of the change determined by political or administrative processes rather than by health characteristics. Difference-in-differences exploits this structure.

The DiD logic is simple but powerful. Compare the treatment group's outcome before and after the policy to get the within-group change. Do the same for the control group. Subtract. The first differencing (before vs. after) removes time-invariant differences between groups. The second differencing (treatment vs. control change) removes common time trends. What remains — the difference of differences — is attributable to the policy, provided the parallel trends assumption holds.

Consider evaluating a state-level smoking ban. You observe lung cancer rates in the ban state and several non-ban states for years before and after implementation. The ban state may have always had higher cancer rates (population differences) and cancer rates may have been declining nationally (secular trend). DiD removes both: (ban state change) minus (non-ban state change) = policy effect. If non-ban states' rates declined by 3% and the ban state's rates declined by 8%, DiD attributes the extra 5% to the ban.

The parallel trends assumption is the backbone of the method and deserves scrutiny. It states that without the policy, the treatment and control groups would have experienced the same change in outcomes over time. This is about trends, not levels — groups can start at different baselines. The assumption is supported (but not proven) by showing that pre-intervention trends were parallel. Event-study plots are the diagnostic standard: they show the treatment-control difference at each time point, with the intervention date as reference. Flat pre-intervention differences support the assumption; diverging pre-trends undermine it. Extensions like triple-difference (DDD), synthetic control methods, and staggered adoption designs address complications that arise when the simple two-group, two-period framework does not fit the data.

Practice Questions 3 questions

Prerequisite Chain

Counting to 10Counting to 20Understanding ZeroThe Number ZeroCounting to FiveOne-to-One CorrespondenceCombining Small Groups Within 5Addition Within 10Addition Within 20Two-Digit Addition Without RegroupingTwo-Digit Addition with RegroupingAddition Within 100Repeated Addition as MultiplicationMultiplication Facts Within 100Division as Equal SharingDivision as Grouping (Measurement Division)Division: Grouping (Repeated Subtraction) ModelDivision: Fair Sharing ModelDivision as Equal SharingDivision as GroupingBasic Division FactsDivision Facts Within 100Two-Digit by One-Digit DivisionDivision with RemaindersRemainders and Quotients in DivisionDivision Word ProblemsIntroduction to Long DivisionFactors and MultiplesPrime and Composite NumbersEquivalent FractionsRelating Fractions and DecimalsDecimal Place ValueReading and Writing DecimalsComparing and Ordering DecimalsAdding and Subtracting DecimalsMultiplying DecimalsDividing DecimalsDividing FractionsMixed Number ArithmeticOrder of OperationsInteger Order of OperationsVariable ExpressionsCombining Like TermsOne-Step EquationsTwo-Step EquationsSolving Multi-Step EquationsEquations with Variables on Both SidesAngle Pairs: Complementary, Supplementary, and VerticalParallel Lines and TransversalsCorresponding AnglesAlternate Interior AnglesTriangle Angle Sum TheoremExterior Angle TheoremTriangle Inequality TheoremSimilar Triangles: AA SimilaritySimilar Triangles: SSS and SAS SimilarityProportions in Similar TrianglesRight Triangle Trigonometry IntroductionTrigonometric Ratios ReviewRadian MeasureConverting Between Degrees and RadiansThe Unit CircleGraphing Sine and CosineGraphing Tangent and Reciprocal Trigonometric FunctionsDerivatives of Trigonometric FunctionsAntiderivativesIterated Integrals and Fubini's TheoremDouble Integrals in Cartesian CoordinatesDouble Integrals over Rectangular RegionsDouble Integrals in Polar CoordinatesDouble Integrals: Definition and SetupIterated Integrals and Fubini's TheoremDouble 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EnthalpyHeat Capacity and CalorimetryEntropy and Molecular DisorderSpontaneity and ΔGEntropy and Gibbs Free EnergyChemical EquilibriumAcid-Base ChemistryOrganic Reaction Mechanisms and Arrow PushingElectrophilic Addition to AlkenesAromaticity and BenzeneDNA StructureCentral Dogma of Molecular BiologyThe Genetic CodeDNA MutationsDNA Repair MechanismsCell Cycle Checkpoints and Cancer PreventionMitotic Spindle Checkpoint and Chromosome SegregationKinetochore Structure and FunctionMitochondria: Structure and FunctionCellular Respiration OverviewBacterial Metabolism OverviewAntibiotic Resistance MechanismsInfectious Disease EpidemiologyFoundations of EpidemiologyMeasuring Disease Frequency: Incidence and PrevalenceEpidemiologic Study DesignsStudy Design in BiostatisticsSurvival Analysis: Kaplan-Meier EstimationLog-Rank Test for Survival ComparisonCox Proportional Hazards ModelCausal Inference Methods in BiostatisticsDifference-in-Differences in Biostatistics

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